CR70736

CRIMIC CR70736

Gene Pld3

CG9248

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	2L:21158847..21160903	39A6-39A7		Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CAGCTGACTTTGTTTTGCTGCC	CGAAGACGACAAAGGCGTTC

Images

Construct Design Sequences

sgRNA sequence	GTCAGCAGGAACCAATGGAATGG
sgRNA2 sequence	AGTTAAAATAACATTGGCATCGG

left_200bp	right_200bp
GAATATTAAACAATAACTATTATTTGTCATTTTTAAACACCCATATTGTAAGGCATTATAGTACTTTCAGGTACTTTTGTTCAGGTAAAAATTTAAATATTAATAAAGAAAATGAATATAAAGAAAATAAAGGAGATGGGGTTCTCCACACACGTCAGTTCCCAGTTGTGAAATGTAATGACTGTCAGCAGGAACCAATG	CCAATGTTATTTTAACTATTTTAGCTCCCGACAATTGTTCAATCCTATTCATTGCTTTTATTCAGTTTTGATCATGCGTGAATTCAAATAATACGCGAATTTACGAATTGGAATAATGCAAGTATTTAAAGTATGGAGATACAGTGAGCTTGAAATCATAACGTAATAAAATTCATTCTTTCATATCCTGCTCGCAGTCA