CR70737

CRIMIC CR70737

Gene Npc2g

CG11314

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	3R:30744605..30745263	100A3-100A

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	multi	AAGGAATGCTGCTGATATGAGTG	TGATAAGACCCGTGGAGGAAAGCT

Images

Construct Design Sequences

sgRNA sequence	AAGTGCCTGCATTCCGTGTCTGG
sgRNA2 sequence	AAATGCATTCGCCACAATCAGGG

left_200bp	right_200bp
TTTAATATTTGATCCGAATTTTACAAAAACGTAAAGTGCCGTCTCCGACACCCTTACAATTCTAAGTACATTGCGATAAGGAGCTCGAATCTAATCAGCACGCCTGGAGCCACAGATGTGACCCAGTTCACGGGTCAAACGGGTACGTAGATAAGCCCGGCTTTGAGTCCGAGTCCGAAGTAGAAGTGCCTGCATTCCGT	TTGTGGCGAATGCATTTATTTTCTGTTAACGATTTGGTGCGTTTTTTTTAATTATAAGTGAGCCGGCTTACGGCCCACTGGGGAAAACCTTAAGGAATAAAGATATTTACTCCAATAATCATCAACCTGCTGGCGACTAATTTGAACCCCCGGATGCCTCGGGCAGAATGAAAATGTATAAAACTTTGATTGAACTTTTG