CR70738

CRIMIC CR70738

Gene Npc2h

CG11315

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	3R:30745479..30746290	100A3-100A

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GGCTGTTGATAAGAGCTATTTAAGAACTCG	CTCCAGGCGTGCTGATTAGATT

Images

Construct Design Sequences

sgRNA sequence	GATGGATCGAAAGTCGGACGCGG
sgRNA2 sequence	TGGATGCTCCACTTAATATTTGG

left_200bp	right_200bp
AATAAACTTTGGGAATTTGGGTATATTACAATTTTTGCTTGCTTAAATTTTGTGACGTTTTAATACGAACGTCATCCGGTGTTAGAAACTATTTTGAATAATTTGAATTACGTAAGAAACATTGGCATGCTCCATTCTCTAGAGTATAGCATTCTATCGCCACAATCCAATAGACTTTAGCCACTACGTGCATTCCGCGT	ATTTGGATTAATACATATCTTAAATATCTTCGAAATTAAAGTGATTTACAAGTGGGTCGATACGATAGTTTGGTTTGGTGTAATTAGCGGCACCTGTTCATAATCTGTATACCCAGTAATTGGACTCATTTCACCTTGAATATGTCAGCAGAACAAGGAATGCTGCTGATATGAGTGCCAATTAACACTTTGAAATGACT