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CRIMIC CR70739

Gene O-fut1

CG12366

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	2R:14161430..14163054	50E1

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CTGTGGGAAACATTTGGGCTT	GCAAACAAGTGTTTTCATAAATGCGAGG

Images

Construct Design Sequences

sgRNA sequence	AAAGAGACAGAGTTGCCACCTGG
sgRNA2 sequence	TAATAAAACTGTCTAGTATTAGG

 

left_200bp	right_200bp
CTGATTAGAAAATAATAGGTTGCCAGATCTGAGCTTTTTAGCCCATAGTAAGAAAGTCTCACAATAAAATTAAAAAATAATTTCACAATATAATTTCATAAATTTTCAAATATATATTTATGTTTGCAATTATATTTATATCACATCATAAACAAAAGCATTCCAAACATTTTGTAATCTGGCAAAGAGACAGAGTTGCC	ACTAGACAGTTTTATTACATTGATTAAGCAATATTCTGGACTTCACTTCTTAAATGGTTAAGGTAAAAGTTTCAACTTACAAATTTAATGAATTTACTTATGAATGTTACCAATATTTAGTGCTTTTAACGAATATCATTATATTTATCTATTATTCTTTTAGCTTTAATTCTTAAAAAGGATTTTAATGAAAACCGTTA

 

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