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CRIMIC CR70740

Gene rush

CG14782

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	:1478552..1481420	2B1

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GAGTCATGACGTTTCGGACGG	CCCAACTTAGAACTCGATAAGCCTC

Images

Construct Design Sequences

sgRNA sequence	AGGCAGTCTGTCGTCACAGTTGG
sgRNA2 sequence	CTATGTGGTCCGTTCAACAGTGG

 

left_200bp	right_200bp
TTCCGGAACCAGGATCCGCCAGTCGCCCCACTTCCTCACCGTTTGCCAGGACCTGCAACAGCTGCGCCCAGTCCGCACACACCGAAGCACGCACGTACACACACACACACATTTCTGGAGGAGCTGTGGCACCGAGTGTCACACCATACACACACGGCGCGAGCGCACGCACACCACTGCAGCAGGCAGTCTGTCGTCAC	TTGAACGGACCACATAGCGAATGATTATGATTATGGTAGATATCGCAGAAAAGCTCTCACCCACCCACAACGCATATCCCGATACCTTGTAGACACGAATCGCGGTGTGACGAAGTCTCCTTGAACATGCCCAACATAGCCACTGTACATTTGAACAACCGACGATTCCCGTCATCTTCATTGCAAGACCTTCGGACCCA

 

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