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CRIMIC CR70741

Gene Sil1

CG10420

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	3R:25233636..25235529	96C7-96C7

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CTTGGAATGTCCAACAGCACAATC	GGTCTGTAGTTCTATAGGGGTGG

Images

Construct Design Sequences

sgRNA sequence	CAGATTTCAGACTTCTGATGTGG
sgRNA2 sequence	CAATCATGCCAAACATCTACAGG

 

left_200bp	right_200bp
TATAGTTTACACTTTTTCAATACTTTAATATATTCTATTGTTTATAACGAATTTATTGATTACAAATATTTTCAAATTTGAAATTGAATTGTAACCGCCTTTGACTGGTTCCGAATCGACTGGCTCTCCACGGAACATATCGCCACATGCGGTATAATCAATGCAGCTCCCTACGGTCATACTATTAACCACATCCACAT	TACAGGATTTTCGTTATCAAATATAGTCTCTTACTTAAGATTATTTTAAGTGTGATTTTTTTTTAATAGGCAGACGATTCACCAACGAGCACATCAAAATAATTATGTTGTTACACGTCATTAAACTAGCAAAAATTTGTATGCTACAGAATTGTTCTCCAAACAGATTATGAATCCTTAAAGCCTTAAGTTCTTGTTTA

 

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