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CRIMIC CR70743

Gene Dph5

CG31289

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	3R:22582028..22583225	94B5-94B5

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GAGTAACATAGGTATCATACAGCTGAG	GCATGCGAGGTCTCTACTATTG

Images

Construct Design Sequences

sgRNA sequence	GGTAGAACATGCTGGGCGACTGG
sgRNA2 sequence	TAAATTAGGTAATTTCGCAGCGG

 

left_200bp	right_200bp
CCAATTAAATGAAATGGCCTTCAAATTCAAAATACGCGCCATTGCTGCACAGGATTTTTTAGCCCATTGTCGCAATGTGAGTATTTCTTGGCGCTCTGGCGGCGGTCACACAACGCGCGACGGCACGTGTTGACTGCATTTAAAAAGCGATTTTAATAACATTTGGCAGGAGTTGCAGTGGCCGAGCAGTCGCACCAGTC	CGAAATTACCTAATTTAGTGTCTAGGAAATGTTGTCTGTGCATCTGAATTGAATTTACGAAGCAAGAGGAGCTGGAAGCTCAGGAAGAAAGTGATATCGCTTGATGCCACCCGTATACGTATGTAGCCTGCCCAGCGGCAGCTGAAATAAAACCGCATTCACCCATCGTACGATTATGTGTATTTATCTCCGAAGCTTTA

 

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