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CRIMIC CR70748

Gene Snmp2

CG7422

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p0	3L:7733250..7743041	66A11-66A1		Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CAGCAATGGCAAACAATCGCACTC	CAGATCTCAAGGTCAATGCACAC

Images

Construct Design Sequences

sgRNA sequence	GATTTGGGACAAGAATGTGCTGG
sgRNA2 sequence

 

left_200bp	right_200bp
CATTCTGACCCACATTTTGGGCCAAATAATTCACAAACTATGTGATGTGATGAATTTCGAAATTTTTGTTTTAGAAATTAATAAATTAAAACTAAACTTAACAAACGTTTAGTTTTATACAAATTAAGGGAACATTAACTTTTTAGCCAAACCCTTGGTCAATTTGATTGAAGTTACGCATACGCCCCTTTGACCCAGCA	CATTCTTGTCCCAAATCCAATCTAAAGCGAATTTCGAATTTTAAACACGTTTCGCTTCGGCCTGTCTCTTTGCCAAATCCGCTCTATTTCGCAACAATTATTTGTATTTTTTTTTTTTCTGAATGACTTTTGGGCCATTGTCAGCCGTCGGCTGAGCCTCTCTTTGGCTCTTGAGGTTAATCAAACGTTTTGTATGCTCT

 

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