CR70750

CRIMIC CR70750

Gene Vps2

CG14542

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	3R:26052821..26054128	96F10-96F1

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CGACTACGTCAAAGATCTACGTTAAA	GGAGCTAGGCAAAAGTTATTCTGG

Images

Construct Design Sequences

sgRNA sequence	AGTTAAGATATCCTTAAACATGG
sgRNA2 sequence	CAGGCAGTGGTTCTTAGTAGCGG

left_200bp	right_200bp
AAGTTGAAGTGTGAACAGTTCTTTCAGAGTGCACCGGCAAAGCTTCTTCTTCCCACTATCACTATTCGCATGTAAGCGCCAACACATCTTGGCCACCTTAGAATCGCGCACACCCTTCGCTTATTGTTTTGGTTGATGAGTAATCTTTAGGAAACTCCGTCATTCTTCGTAATTAAATCTTGCAGTTAAGATATCCTTAA	CTAAGAACCACTGCCTGTCCACTTTATGTAAGAGCCACCACCCGTTTGTATCTCCCAGAAGCGAATGTTATATTAAAGAGAATGCTTGTGATTTTCGTTCCCTGCGTGTTTGAAAAATCGATGAGTAATTAGCATTTACAAGCTATAATGGAAAGAACTGGGTAGTGCTGCAATCGCACAGCCTAGCTACAAACAAATTC