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CRIMIC CR70752

Gene wol

CG7870

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p1	2L:8301345..8302985	29A3-29A3

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CTACAAGACGAAGCCCTATCCC	CCGTGCTTTTTCGAGTAACCC

Images

Construct Design Sequences

sgRNA sequence	AAGTTTCTGTAGATAAATTCGGG
sgRNA2 sequence

 

left_200bp	right_200bp
AGGAGACCTTCCTGGACCCGCACACCATTAAAACAGTGACTTTTCCCAGTCTCGAGGACTCGCCAAGTTTGGAGCTCAGTGTCATCGTACCAGCTTACAATGAGGAGCAGCGCTGTATGTTGCTTATTTGTTTTTGTTTTGGTGAATCACAGTCAACATTTTATCTATGCCCATTTGTGATTGATTGTCTGGCACCCGAA	TTTATCTACAGAAACTTCATTACACTAACATGTTACTTTAAGTTACTTTCCCCAAAAGCTATTCGATTTTCTTGTTCTCATTTGTGTGTGTTTATACTCGTTTAGTGCCTTCTATGCTGGACGAGTGCTTGGCCTTTCTGGAACAGAAATCTGCTGGCACTCCCAATTTCACATACGAGGTCATCGTGGTCAGTGACGGC

 

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