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CRIMIC CR70753

Gene mys

CG1560

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p0	X:8061645..8070237	7D5-7D5

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GCTAATTGCACAGCAACCTATG	CTCAGGTAGCTAAGGGCATTGAATTC

Images

Construct Design Sequences

sgRNA sequence	TTTATGTTGGTTATGGATAATGG
sgRNA2 sequence

 

left_200bp	right_200bp
GCATCCGAAGCATTTAGCCTCAAAATTAATGACCTAACATTGTTCATATTTCAAGTTTTGCACTTTGCACATTTGTTATTGCACGAAAAATGATTATTATTATTATTTGTTTGGACGCCGCCTGCTCCTTAACTCTGTGATGTATTGTAGCAAACACCACATAGTCATCTATCTAATCTAGCATTTATGTTGGTTATGGA	TAATGGTTACATCTTTTTTGCATGCATGCTTTGGCTTTTCTGCTTGGCTGGTTGTATATGTTTGTAGTTTGGACTCTTATCTGCTGACACACTCTGAAAAAATGTATTCAAATTCTTAATTGGAGAAGAACCATGGCAGGCTGAACTGAAAATGTGAAAAAATATTAAACCATAGAAAACGACTGCCTAATTGACGATTA

 

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