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CRIMIC CR70755

Gene BNIP3

CG5059

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p2	3L:20514598..10519393	77C6-77C6

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	ATAGCCCCAAGAGTCCACC	CTGGTGAAGATTGCTAGAGACCCT

Images

Construct Design Sequences

sgRNA sequence	GAATGACCATTTGTGGTAAAAGG
sgRNA2 sequence

 

left_200bp	right_200bp
TAAGAGATGAGAATCGAGGCGGAACCGAGGTGATCTTGCTAACAATGCCAGTCTCTGTTTTTGCTTTTGCCAGGGAGGAGACAAACAGAATGCAGGCAATGAAGATTGGCTGAAGAACTGGAATCAGCCACCCAGGTATGAAAGTTTATTTAGCTAAGTTTCTAGAATCTAGAACTAAGTTTGGAGCATTGCGGCCTTTT	ACCACAAATGGTCATTCATTGAATTATTATTTACAGTAGCTGGAACATTGAGGATTCAAGTCGGGATGCCGGCGACGAGGGCGAGAAAAAGAAGACTAACACAGGCTACTCCATCCGTTTGAAGCGACTGGGCTCCAACTCGTTGTTCTCCCGCGAAATTCTGTACTCGCTGCTGGTGACCAACGTCCTGTCCCTGCTGC

 

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