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CRIMIC CR70756

Gene Fur2

CG18734

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p0	X:16373086..16381782	14C1-14C2

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	ACAGTCCGTATAATCTTTTACGCC	CGGGATGGTTTGTCTGAATGC

Images

Construct Design Sequences

sgRNA sequence	CAACAAAATCAACACTAACACGG
sgRNA2 sequence

 

left_200bp	right_200bp
TCAGCTCGCATTTCATCTTTCCGGATCCGTTGTTTAAGGAACAGTGGTATCTGGTGAGTAAAGTATGTAATCATCTCTCATTTAAATGCATGCTTCATGAATCCATATATATTTTATAGTCCATTAGTCTATTAGCTCGATTCTCTATACCATCAGCGTAGTTTCCTTTGAGACGTTCAATCGATTGCTACTAACCGTGT	TAGTGTTGATTTTGTTGATTTCGAAATATTTTCTTTTCTTTTAACATGCTTAATTTGTAAATGTTTGGATTTCTTTTTTGATTGATTGCCTATTTTTTGCTTATCTCTTCGCCAATGTCATGTTTGATAATCAATTTGTAAATTCCCACAAAAATCGCTTTGTAGAATGGCGGCGCCAAGGATGGCTTGGACATGAATGT

 

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