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CRIMIC CR70757

Gene EloB

CG4204

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p0	3R:20578222..20579850	92F2-92F2

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GTGGTGCAAGCGAAGTAGG	CATTCGCTTCAGCTCGGC

Images

Construct Design Sequences

sgRNA sequence	TAAGAGCGCGACGGTGTCTATGG
sgRNA2 sequence

 

left_200bp	right_200bp
GCCCGCGACCGCCAAAGGAAGCGCCACCCACGCCGTCAGTTGCCGAACACAGCGCACTAGCCCACGCACCTGGTTAACCCACATCCGCGTAGCAGCCTCAGTCGCAGCCAGCGCCACAAACTCCTCAATATGGTGAGTGCTCGTGCCGGTGTCGTGCAGCCTAGCAAGGATGCAGTGCATCGCAGATGCTGCTGCCATAG	ACACCGTCGCGCTCTTAATGCATGTGCACAAATGTCGCCTGTGTGATGGCAAGATCAGAAAGCATGACACACTGAAAAAGCGCCACTGAACTTTGACCGAAACGAATCACTTCGCATCGAATCAAATCTAATGGCTAACCCTCTCTTTTGCAGGACGTGTTCCTTATGATCAGGCGGCAAAAGACCACCATCTTCACGGA

 

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