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CRIMIC CR70758

Gene Npc2e

CG31410

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p1	3R:10053332..10054348	85F8-85F8

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GCTGCGAATCGTTGTAACTTTGG	GATTCAAGTGGACACGGATATAGATGG

Images

Construct Design Sequences

sgRNA sequence	ATCTGTTGGCCAGGTTGATATGG
sgRNA2 sequence

 

left_200bp	right_200bp
TGTAAGTAGCTAAAAACTCTTTTATTTTGTTGGAAAAACATCAAATCATTTTAAATCGACCAGTTTGTTAGATTGGATTCATGATTCTAACAGATTTCTATGACGATGATTCACTTCGCAGACTTTGTCTCGAAAACTATTGCCAAAAATACGTTCTGAGAGAAATTTAAAGTCCTAACGTGTATCTGTTGGCCAGGTTG	ATATGGCTTATCAACAAACAGCTAATCTCTTAGAAAAAAAAACATAGCCTTCTTTTGAGCTCACCTTTTGATTCTTCGAAAACATATTTCCGCTTTCAGGCAAAAATAAGCCGTTTCCACTGGATGTGAATATTAAGGATTGCGAGGAACCGCCGTGCGTTGTGTACAAAGGAACCATAGCAGTCATGGAGGTGCACTTC

 

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