CR70760

CRIMIC CR70760

Gene UVRAG

CG6116

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	2L:13286777..13289556	34B4-34B4

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CGCGCTTCTCAACACTAGATTCTC	CGATGTGTTTCTAATGTTTGTCATCCAC

Images

Construct Design Sequences

sgRNA sequence	ACGCCTTTCCGGGTGAGCTGCGG
sgRNA2 sequence	TGGATATTGGATATAAACGCTGG

left_200bp	right_200bp
GAAAATAGCCAAATTAATAAAATACAAGTTGAACGGCGCATTTCCCACCACTATTGCCTTCAGTGTTTTGCAGCACTTATGCATCTAAAAACAGCTGTTCGGGTAATCATTTTTTTTGTTAAAAGTTTCTGTGGAAAAACTATAACAAACGTAGTCTTAGTGTACAATGATCTGGGCTACGGGCTCCGCGTAATCCGCAG	TTTATATCCAATATCCAAGATAGCATTGTTTAGCGGCTCCACCTCAGCGGGTTTCTTGGTATTGGTCGCGTATTGTATACTTTTAAATTATAAAAGTCAATGGTGAAGTATATGTGAACGGTAGTCCATTGCGATAATATTATTTTTAGTTGTTTAAGATCTCTGTGTAAATTCCCTTTTATTGTTTGTTTGCTAATATA