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CRIMIC CR70766

Gene ptc

CG2411

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p0	2R:8649649..8665223	44D5-44E1		Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CACTGTAGAAAGACCGATTCCGC	CGAAGAAGACATCGGCGACGAAAA

Images

Construct Design Sequences

sgRNA sequence	TATTCTTGAATCGTATTCGGAGG
sgRNA2 sequence

 

left_200bp	right_200bp
GCGACAGCCTCCCACGCGTTCCGGACACACACGGCGATGTGGTCGATGAGAAATTATTCTCGGATCTTTACATACGCACCAGCTGGGTGGACGCCCAAGTGGCGCTCGATCAGATAGATAAGGTGAGTGCCCAACTACAGTGAACTTTCACTGTGAAGGATAGCCATGTGTTGAATTCAATAATATTCTTGAATCGTATT	CGGAGGATCCTTTATAAGTTTAAATGGAAAATCTGGCTCTTTCGAAACTTTTGTTAGTAATCCCTTTTACTTTAAATTAAGCAAAAGATTTGAAGTATTAAGTAAATTTACAATCTTTTTAAAATTTGAAATTGTTATATTGTACAATTCTTGTCATTAGAATATTGAGACACTTAACTAAAAACCATGATGTAGAAGTT

 

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