CR70770

CRIMIC CR70770

Gene CG11617

CG11617

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	2L:204743..207297	21B4-21B4		Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CATCTGGCTCTCGAGCTCTC	CGCCAAAGTCTACGTAGAAGTC

Images

Construct Design Sequences

sgRNA sequence	GCCGCGTTGACTTGTCCATGAGG
sgRNA2 sequence	AAACGTGCAATCTAGTCAAGTGG

left_200bp	right_200bp
ACAAAAATATTTATTTCGTTATTTTGAGAGCAAGAAATATCCACAGAGCAAAAAACATGAAAATCGGTGCGCCAGAAATAGCGCCACCCACTGAAAGTTCAAAAACTAAATTGACTTACACGGTATTTTTAACAGAGATAGCGATGTGTGCGATTCTGTGTCAGTTCTTTAAATTACTTTCCTTGTTTTCAGAACCTCAT	AAGTGGGCTTCTTCTGGTTTTCATTTTCGAACTTTACAGTACAATACTCACTCGTTACATGATTTTCTTCAAGTTTAAAACCTTTTAGCTACACTCATTTAAATTATGTGATAAACTACGACGATCATATTCATGTAAACGTGAAAACCAGGTTGCGCCGAGAGTTAAGAATTACTACAGCTAGTTTAGAGTGAAAAACA