CR70772

CRIMIC CR70772

Gene CG11710

CG11710

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p2	X:20427411..20429673	19D1-19D1		Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GGAAAATGGTTGCTAATGGCTAATTGG	CAATGACAATCATTACCATCCAGAGATACAC

Images

Construct Design Sequences

sgRNA sequence	ATGTTCAGTATGGCCCTGGAAGG
sgRNA2 sequence	TTTAATTTCTCAGAAACTCTAGG

left_200bp	right_200bp
TTGATTTTTTAAAGTTTTTACCATTTTATTTCACAATTGTAAGCAGTTGGCAAACTTTGGGACTGCGAAATGGAGAATTTCCTGCGGGGCACGTTGTCCAAATGTCTGGACTGTGTAATCACGGATCAGATGTTGGCGTAAGTACATGATGCATCCAGATACGAGTAAATGAGTTTCTCAATGGCTTCATATGCCCTTCC	GTTTCTGAGAAATTAAAGTGGATTGGAATATTTTAAACATCTTGTAATATTGGAAAATATCATGCAATACAATACGAATTAAATATTGAATTTTTTAAATATTAAAGTAGTAAAAATATCGTGTAAAACTATAATTTAGCAAAAGAAATTAATGTAATAAAATACTTCTTTGAAGTTTCCTTCTTTGGAAAGTTATAATC