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CRIMIC CR70776

Gene sug

CG3850

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p2	2R:12924567..12930644	49E1-49E1

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
no band	OK	GCAACTGGACAGATTGCGATAGG	GCAATAGTAGAGTCCGTCTGTCAG

Images

Construct Design Sequences

sgRNA sequence	AGCAAATGCCAGGCTGCCTCCGG
sgRNA2 sequence

 

left_200bp	right_200bp
AAATTTCCTAAAGGGGTCTCAGCACTTTCGTAATGTTTTTTAAAAACGAAAAAAAAACTCACCATTATATTTTCTTTTGAATTTTGAGTTGCCAGAGAAAGACATTGAGTAGTTCTAGAAAAATATAAAACACAGTTCCCTCGCGTTTTACCATTGAAACTGATTACGCATTCGAAAGAAATAAAAAAAAAACCCCGGAG	GCAGCCTGGCATTTGCTGAACTGTTGGACTCGACTTTTTGATAAACCCTCCAATAGCGATAATAATGTGATTTTGGGTCAAATTGATAGAGATGCAGTGTATTGTTTATGGTCAGCAAGTGTATTAATATTGGATAAGAAATCAATCCAGTACATGTTATTTTCGTACTAACCACTTATATCCCTTTGATCCTTGCAGAG

 

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