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CRIMIC CR70777

Gene toe

CG10704

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p1	3L:12422510..12432668	69C1-69C1

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CGCTTTTGCAATTGACTGAGGC	GAAATCGCAGAATTGCGGAATGG

Images

Construct Design Sequences

sgRNA sequence	TAATTAAACCCCATGCAGATGGG
sgRNA2 sequence

 

left_200bp	right_200bp
TCCGGGACTGGGGCAATCCGGGGGCAAGCTCACCGCCTGTTGACATCCAAGTTGGCCGGCTGTTGCGGCGGCATAAATCAAGCATGGCCATAACCGCGGCCACAACAATTGGCGCAACATGCGAGTGCAACAAATTGCAGCCGGGCACTTTTAAATGGAGTAAGCTGGTCCAGGTCCAGCTCCCAGCTCCCAGTCCCATC	TGCATGGGGTTTAATTAGCCCAGGAGCTGGCGAAGCTGGAGGAGATGCCCCAATCGAAGTGCATACTGGGAAAACGATGGCATGCCGTCGTCGCCACACACGGAGCTCGATCGCACCAAAAAAGAGGTGTTAAAATGTGTGCAGTTCTCGCGATCAGATAAAATGTCAAAATGAACATAAAAGCTTCTACATCCGAGGAA

 

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