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CRIMIC CR70778

Gene CG14655

CG14655

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p0	3R:4777497..4780302	82D5-82D5		Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TTTCTATGCTCACTGGCAGC	GCAGTAACTGCACTGGTGGTAG

Images

Construct Design Sequences

sgRNA sequence	ATTTGAAACTGAGAGACAGACGG
sgRNA2 sequence

 

left_200bp	right_200bp
TACCCTTATATTTTAAAAACAACTGATGTATGTACGTATATCGTTCCCATTATTTTCAATTAATTTTTCAACTGTTCATATGACTATGTATATATATGATAGATATTAATTTGTTTTTGATCAATTTAAAACCATGGCAACTTTGTGATATAGTGAATGAAAGTGAATGATTTTTGTGCATAAATTTGAAACTGAGAGAC	AGACGGTAATGTAATGGAATATTTGGCCATGAGGGCATATTGATGAATTGATAAATATAAGTGAAATTCCGATTATTGCTTTATACAGGACCAGGAGCCCTACAAGTGCCATCTCTGCTCCAAGACGTTCCGAATGAAGGGCTCCCTTCGAATCCACCTGAAAGTAGTGCACATGATGGGCGTGCCCTGCTCCAATCCCA

 

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