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CRIMIC CR70779

Gene hng3

CG13897

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p1	3L:737680..755489	61C9-61C9		Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	ACACGTGCAGCAAACAGACGGTAA	CATTTGGTTCGGCAAAGCTC

Images

Construct Design Sequences

sgRNA sequence	CGAAATCGGGATCAATATTCGGG
sgRNA2 sequence

 

left_200bp	right_200bp
GCATTTTGCTCAGTTCGCCAATAAAGACGCTGCAACATGGACATGCACAGGCTGATCTTCGAGGTGCAGCAGCGCCGGGCTCTCTGGGACGCGAGGCATCCGGACCACGCCAACCGACCGGAGACCCAGCGCCTCTGGAACGCCGTCGCGGAGGCCACCGGAATGGATGGTGAGTGCACTGAGCGAAATCGGGATCAATA	TTCGGGTCAACACAAAAATCCAATTGGAACTAATTAAAATTATGATAATTTTAGATTAATTAGAAAATTGTTAATGCGAAGTACTAGTATTATTTTTTAGTATTTTATGGTTTTTTCAAATTAATTATTACATTAGTATTAAACTTTTTTCGGTGTACTTTGTAATGAGTGCTTTGTTTACATTCTCTTTTCTTAATCGC

 

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