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CRIMIC CR70781

Gene Bug22

CG5343

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p0	2L:10429920..10430918	31E1-31E1

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GGATAAGAAGGTGCGCAATGG	GCGGAGCAGAAATAGCAGTG

Images

Construct Design Sequences

sgRNA sequence	GTCATCGAGAACCTGATTAGGGG
sgRNA2 sequence	ATCGTCGACTCCGTCTGGTTAGG

 

left_200bp	right_200bp
AGAGCCTCGTGTTGGAAATAGTCGGCACCAATGTCAGCACCACGTTCATAACATGTCCGGCAGATCCGAAGAAGACGCTGGGCATCAAGCTGCCCTTCCTGGTGATGATCATCAAGAACATGAAGAAGTACTTCACCTTCGAGGTTCAGGTAAGCTTCGTCATCTTGCATAAGACAGTAACTCCATGATTTCCGCCCCTA	CAGACGGAGTCGACGATTAAAACGGCACAAAATGCACAAGACTGCCATCCAGCACTCATTCAACACTTTATGGGAATCACGAATAAAAATTGCATCATGTACATAAAATTCTATTGATCTCTTGTAAGTATCTCCGTTCCCGTCTCGGTTACCAGAATGGTGTGCTCGAACTGGGCACTACGTGCGCCATCCAAACTAAT

 

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