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CRIMIC CR70787

Gene Inx2

CG4590

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	X:6998672..7004151	6E4-6E4

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
no band	OK	GTTGTCGCGTTGGTGTTGAG	CTCTCTTACTCGGTGAAAAGTGTTTC

Images

Construct Design Sequences

sgRNA sequence	AACAGTCAGCGTTAGTGTCAAGG
sgRNA2 sequence	CTTACTGGCACTCTGGCACACGG

 

left_200bp	right_200bp
GGGATCCAAGCCGAAGATCCAGTGCCCAGCCAAATCCCACGTATCGCTGCTCTCGCCGATGGTTACTACTATCTTTTTTTTTCGTGTGCATTCTTTGAACAGAAACTGAAGAGTAATGGTTGCCAACGAGTGAGGAACCCGAAAGTCCCGTAGCAAAGTCACCAGGCAGCCGATACGATCCGCAACAGTCAGCGTTAGTG	GCCAGAGTGCCAGTAAGTCTCGTATATACCATCCCACACTATCCCAAACACTTCCCCATCATCATCCAGACACTAGTTTTAGTTAGCTTCTAGCCCAAGCCACAATTCCACTCGATGTAAACCTCGATTAGATCTTAAGATTATACATACGATATACCATTTTTGTTTTGGTAGTTAACTTACTTTTTTTTCGACGCTAC

 

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