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CRIMIC CR70791

Gene shakB

CG34358

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p2	X:20761071..20927050	19E3-19E5

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CAGCTCTCTGACTAGCCAAATGTAC	TGGCACATGACGATCTCAGG

Images

Construct Design Sequences

sgRNA sequence	ACTCGAACTCACCTTTGTAGAGG
sgRNA2 sequence

 

left_200bp	right_200bp
TCCAGTTAAATGAAAATTACCAGTAATAGAAAAAGTGCAGATAATTTTCCAGTTAAAACCTTAATTACCCGATATATTATTTTCAATAAACACAACCAATGTAAAGCATCGAGTTCAAAAGAATAAGCCTATCTATTAAATGAACCCTTCACAATTTATAATGGAATTCAGGCAGTACAGTGCACTCGAACTCACCTTTG	TAGAGGATTTCCAAAACAAACATTTCCGGGTAACTCGAGTAGTCCCACTAATTAACAAAGAATTATCCGGAAATCGACTGTGAGTCACGAGCCAGAGTTCTTCAGAGACAAAGGATACTCCGCCAAAGGTTTTTGGCATCCATACATATATTGTATATTTATTTACATCTGAATCACAGCTAATGGCTAAATTTCTGGCG

 

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