CR70792

CRIMIC CR70792

Gene zpg

CG10125

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	3L:6604366..6606032	65B5-65B5		Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	AGCTGCAGGCGAAAATACG	ATACTCATACACCACCTTGAACGAC

Images

Construct Design Sequences

sgRNA sequence	TAACTTGTACCAAAAGCACGCGG
sgRNA2 sequence	CAGTATATTGAGATTAAACTTGG

left_200bp	right_200bp
GTTACCAGATGGTAAAAACAGTTTTTAGCCCCAACAGAGCTGGCAGTGCCGCTATTTTCTCGTCAAATCTGACTTCTTTCCAGCCGAAATCAGTGCCTGCCAAAAGCTGGCTGCTGGAAATTGCGTTTTGTACGTAAGGTTCCCAGGCGTGAGTAAGAAAGAGACAACCATAGTATACATCAACCGTTATGATGCCGCGT	TTAATCTCAATATACTGAAATAAGTATTATCATTTGAATCATCTAGAGTAGAACACTAAGATGACACAAACAAGAATAACATACATTAATTTTTTCACATCGATATGTGCATATTATCGAAGATATAACAAACTATTATAATTTTTGTGCTAAGAAAAATGTGCCATTTTCGCGTGTTCAGTAAATTCACATATTTCTTA