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CRIMIC CR70793

Gene bsk

CG5680

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p2	2L:10247502..10250501	31B1-31B1

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CCGATATGCTCCTTGCTCTCAAC	CTGAAACTCTTCCAAGTTCCTTTGC

Images

Construct Design Sequences

sgRNA sequence	TAATCTAATCTAATATACAATGG
sgRNA2 sequence

 

left_200bp	right_200bp
ACACCGTCGAGGTGGGGGACACCAACTTCACCATCCACAGTCGGTACATTAATCTACGGCCCATAGGATCAGGTGCCCAAGGAATAGTATGGTAAGTGGTTATCAATATGTTATTACATATAATACATTTAACAATTTTTTGGCTTAAACAATTAAGATATTTCGCATGAGTATGAAGTCATATTTGTAACGCTCCATTG	TATATTAGATTAGATTAGATGGGATTAGCTGGAATATGAAAAAAAAAAATAACAAATTTAATGCATTTCAGCGCCGCTTACGATACTATCACCCAGCAAAATGTGGCGATTAAGAAACTATCTCGACCATTCCAAAATGTAACCCATGCCAAGCGAGCATATAGGGAATTCAAGCTAATGAAGCTTGTTAACCATAAAAA

 

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