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CRIMIC CR70794

Gene Strump

CG12272

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	3L:16122002..16126052	72D8-72D8

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CAGTTTCTCACTTCAAATTTATGCGCC	GTTCGGTGAGTCACGATGAGAT

Images

Construct Design Sequences

sgRNA sequence	TGTTAGAATCACAGAAATCAAGG
sgRNA2 sequence	TTTGTGTAGCCCAAACCTGTTGG

 

left_200bp	right_200bp
ATAGTGTGACCGGACTTATGTAAGATTACTGCAGATAATACACTAGTGCAAAAAAGAATTGATATGACTAAATGAATAGACAATATAAACAAACAATAACATAATTCGTGGACTGATAAAAAGTATGTACAGCTTTAATTAATAATGTGATTATACAAATTAAGCTTTCTCCAGAGAGGCATTTGTTAGAATCACAGAAA	GGTTTGGGCTACACAAACTTTAATAAATCCGAAACATAACAAGGAAATGCCTCGCTGAGGTACAAGAGGAGATTGTAGTTGGATGGGTTGGTAATCTCAGTGTTCCTTCGCTGACAGGAACTCTTTACGTGATAACCGTTGGAGCGTTGCGTCCAGTGAACTTGGGCAGCTGAGAGATCTCCAGGGCGATGTCGATCAAG

 

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