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CRIMIC CR70803

Gene enok

CG11290

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p2	2R:24099210..24107032	60B10-60B1

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CAAGTCGCAATCCTACGTGC	CAAGTCGCAATCCTACGTGC

Images

Construct Design Sequences

sgRNA sequence	GCATGTCTTACACCTAAATGGGG
sgRNA2 sequence	TACAGCACACATTTATGTACAGG

 

left_200bp	right_200bp
GGGATTGCCAGAACTGCGCGGACTGCGCCGTTTGCAAAATGAGAAATCGGGGCCCGTGCTTACTGCAGTGCTTCGTGTGTAAGGACCACTTTCACTTAACGTGTCTTGATACGATTCCAGACAAAAAGCCGAAGCATCCATACAGGTGAGTCAGTGTAAAATTGCCAGCTCAACTTGACTTTACCGTCTTTATACCCCAT	CATAAATGTGTGCTGTAAATAAATATATATTACGTCAATTTAACAATCCAATCCCCCAGTTTAAAATGATTCAGCGTCGTTGGCGAGTCTGTTGTTTAATACTTTTCTTTATTGCTTAGATCTAACATTGTTCAATCAAAGCTTGTTTATTATTTCGAATCTGCTATAATCTAGACTAATGTCAATGTGTTCTTAGTTTC

 

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