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CRIMIC CR70805

Gene MAN1

CG3167

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_SAKG4	2R:24033974..24036707	60B5-60B5

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GACTGTTGCTTTAACGTCGGTAG	GGGTTTCAGCAAAATCAAGGAGG

Images

Construct Design Sequences

sgRNA sequence	CAGCACACACATGCATACACAGG
sgRNA2 sequence	ACTTTACTTTCACACCAACGAGG

 

left_200bp	right_200bp
TGTTTAGGTATGTTATTTATAAACTGCATTAATTTCGCACATCTTCCCGTGTCCGTTGAATGGTAAACATATTTTTCGCCATTGAATAATCAGACATAAGCGGAAACTTATTGGCCAACAGGCGCCGAAATCCCTCTGCTTATTTCCAAGGGCTTGTGAAAATGTGCTTCGCTATGCAGTTATATATGCACATTCCTGTG	ACGAGGCAGCCAACACTCACTCATAAAACACACAACTCTACATTAGATACTTAAGTTATTAAATGCGAATTTGTTTTACATGTACAGCTGGAGTTGTCGTATTTAATCGATTTATTATGAACTAGACTTTAAAACTTAAAAGCTGCTAAACACTACCACTACTTTGCTAGGGAGTTGGACGACAGAGCGCTTTCCCCAGT

 

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