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CRIMIC CR70807

Gene CG5270

CG5270

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	3R:11422092..11429969	86E5-86E5

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	AGCTCTGCGAGATAATATGTGGG	GGAGGGTGGAATACTTGCTGG

Images

Construct Design Sequences

sgRNA sequence	CTCCTGCTGGTCCTCCATTGTGG
sgRNA2 sequence	ATGTGTTATTGCTCGAGGGTTGG

 

left_200bp	right_200bp
ACAGCTGCTAGTCGGTCAGCAGTGGAAACAAAAGGCGAGCTGTAACACAATAGATCCCGATAATCAGCAGTGGAAGATCACTTGGCGGAGAGCCCCAATTCAGAACAGAGCAGTCTGAACAATGAACTAGCATTAGTACCAGTGAATCAGCAGAGATTAGAAGAACGTTGCACTCCCGAGGAGCCACAGGTGTCCCACAA	CTCGAGCAATAACACATTAGTAACCCCTTGTTTCTGGGATTTACTAGCTGTGAGAAATACCAAATGATGAATTTGTAGCTTTGAAAACTCCTGCATTCTAAAAGACAATAACATATCTAAGCAAATTCTCAATTTTGAGATTTAGATGAATATATATTATTCGGAATTGGAATTCATCTTTTGATTATTCACCTAGTGAA

 

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