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CRIMIC CR70808

Gene GXIVsPLA2

CG17035

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	3L:15955096..15956465	72B2-72B2

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GAATTTTCCCCCCTCGAAATATCG	CAAAGGACCACGTCAGCATAAGC

Images

Construct Design Sequences

sgRNA sequence	CATCGTTCAAATATACCAACCGG
sgRNA2 sequence	ACGAGGGTGCGGGGACCTGGGGG

 

left_200bp	right_200bp
TAGCATTAATTGTAATACTCTCTGCAAAGCCAACAAGATTTTATTTTTCATAAATTTTTTTGTTTTATAAATCATAACGTATTTTATTTATAATATTTAAAAACGGTTTTAAATTTCCCATTGGGATTTTTTGAAATGTCAGGACAGTGCCTAAAAGTATGCCACCTCATATGCAGTTTGGTATGATTTCCGAGCCGGTT	TGGGGGTATCGGATTAAGCGTTAAATGTTTTATGTGTTGTGTTAGTCTATGAATTGGGTAGCCATGTCCATTGGACTCCCTTTTTGACGAATTTCATTATTGTATACCAAATAGTGGCATGCCAACGGTCCAGACTGTTAAATACATTGATATTTTTACCTATTATAAAGATGCTATATTTTTAAATCAACTGTGCGCTG

 

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