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CRIMIC CR70812

Gene CG13609

CG13609

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	3R:24227023..14228535	95F3-95F3

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GTTATGAACACTGGGATGCGG	TATGGCAACGTATGACAGACATTCG

Images

Construct Design Sequences

sgRNA sequence	TTGTCGGATAGGCAACTTGTCGG
sgRNA2 sequence	AAAATATTTATTTGAACTCTAGG

 

left_200bp	right_200bp
CGGTTTTAGCGAGTTTTTAGTTTTAATTTTGTTTTGACACAAACGCAGAAGAAGAAGTGAGAGTGATTGGCCAGGGTTGCATTGCGTTGCATCTCGATGGCCGCCAATCGACCAACCAGCCAACCGATAGTGCGATACCAATAACAAACCGCTAAAAGCAAGAAGAAGCATATCACAGATAAACAAATTTTTAACCGACA	TCTAGGTATATCAAATTTATAGTCATCGAAGAAAGTATTGTTTTATAATTAGATATTTTTTTTTTTTTTGTTTAATTATTATAAATATAGACCAAAGAATATTTAAACAAGATACGCTCGCACGTCCCCAGTGGGAAAACATACCAAGGCTAAAATATGTAATCAGATAAATAATCATAAAACTGCGAACTCCTGAATGC

 

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