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CRIMIC CR70813

Gene Atg9

CG3615

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	2R:16294400..16297232	53B1-53B2

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TAGGCTGTTCACACTTCGGG	CACAGGATACACAACGGATAATGC

Images

Construct Design Sequences

sgRNA sequence	AGAACGGTTGACCTTGACGTTGG
sgRNA2 sequence	TGGGTGGTCCTTAGCTCTACAGG

 

left_200bp	right_200bp
TCCCTGGCAGTGTGAACACTGCACATTGGGCGCTGCAAGAGGTTCGCCTTACTTATTCATTACCCAGTATTTTGACGTTGGCCAACACTTCTCTTAGGAGAGTCAGCTGTTTGCTGAGAAGGTTCAGCAGAATCAAACAACAAAGAATTTTCCAACTTATACTATACAGCGATATAAATAGTCAGAACGGTTGACCTTGA	GAGCTAAGGACCACCCATTCTGCTATTAGTGATTAACCTAAGTTTTAAAGTGTAAACGAACGAACGACGTCGTCTAGTGCGTAGCTAAACTAATCTGAAACTAAGCTAAACTAAACGAGAAGCAATAAACATAAATGCATTATCAAATTGATCGGCTTTATTATTTCAAAGGAAATTTCGCTAGATTTTTACTAAATCTA

 

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