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CRIMIC CR70814

Gene Hecw

CG42797

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p2	X:9608946..9623243	8F1-8F1

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GGGTTCTTAAAGATTTGGCACATCAGT	ATCTCTGCGAAATGGTGGC

Images

Construct Design Sequences

sgRNA sequence	TTGATGCCTCATGGCTAATCCGG
sgRNA2 sequence

 

left_200bp	right_200bp
TTATTAGCTTTAATCAGGGGAATTGCAATGATCTTAGATTTTCTCTCAGTTATCCCATATGATCAAGTCACAAGACCAAAGCGCATTTATCTCCTTAATCCGCTATCAACGCACATACCGTATGTGGATTTGGATTGGTGTCCTTTTCGGCCTGTCGGATGTGTTGTTGCTCTGCCGCGTGCGCTAATCAATTGCCGGAT	TAGCCATGAGGCATCAATAGCACCGCCGGGGGCCGCTGGAGCGAAAGTGACGCCACCAAAAGCGAAGTGTTCTGGCCATAGATTTATGAGTTACTTAATTGCTGTTTGCGACGAGTGCAGCTAATTTGAATTTCTATGTTTCACACAGCCTGTTCGAGCAGGAGATCATGGGCTTTGTTCCTATTGAAGAGCGCAGTCCG

 

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