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CRIMIC CR70815

Gene Cul4

CG8711

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p1	2R:8102260..8106318	44A4-44A4

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	AACATGAATTCTGCGTCCACC	GCATACAGAGCTACTAGTACGAG

Images

Construct Design Sequences

sgRNA sequence	TAGGCTTATCTAAGGAAGGAAGG
sgRNA2 sequence	GTCGCTACACTATTAGCTGGCGG

 

left_200bp	right_200bp
TGCCAAACTTCTCCAAGCTAGGCGGCAGCCATGGCGAGATCCGCACGGCTTCAACTACCTCAAATCTCCTCAATCGCATGGGGGCTATTCACAACAGCAAGCCTGGCGATGTCAAGAAGATAGTGATTAAGAACTTTAAGGGTAGGTCCCGTCTAGATCTCAACTGTTTGTATACTGCATGATTCAACTTCCTTCCTTCC	GCTAATAGTGTAGCGACAGTTCTTTTTGTAAGTTACCTTATCATACAATAAATTAAGCGTTTATTTTACAAGCCGAGTCCTTTGAAATAGGTCATCGCCCTCGGAACGTCGATGCCTCCAGCAATTTAAAAAAGTTTTCGAACCGAAAGCAAACTAGGTGTTCAATAAACTTATTTATTGGACTTATTCTAATTTGTTTA

 

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