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CRIMIC CR70818

Gene Sgsh

CG14291

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	3R:18911053..18912922	91D4-91D4

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GCCAGAGTCATGCAAACCAAACTC	GCATTGCGCAGGTGAAAATCAAG

Images

Construct Design Sequences

sgRNA sequence	GACGTCAGCGTACTGAAGTACGG
sgRNA2 sequence	ATGGTTGAGTTATCAACCATTGG

 

left_200bp	right_200bp
GGTGTGTTGACTGTAGTCAAACCGCTCCCAAAGAAAGTCGGGCAAAGTGAATGTACCTAGGCGCGCTCCCGCTGAGCATTTGTTGTGTTGATGAGATTAAAACTGGAACTGGGAATGGAATTGAATGCGTCTTAAACGGAGAACGGAGAAATGAGAGGCTTTGTGGACACTTAAAAGTATGCGGCTCTCTTGAGCCGTAC	GTTGATAACTCAACCATGCCAATGTTTTTCACTTCACATGCCGTTACAGTTGCGGTCATACAAATAGGAATTGCAAAAGGTAGAAATGATAATATTATATTAATGTATATTCTTATTAAAATAATAAAAAAAAAGTAACATACAATACTTTCAATAATAAACTTTGTTTAACTTTGAAGCGAGCTGCTAATCTTTAAGCA

 

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