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CRIMIC CR70820

Gene IntS1

CG3173

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	2R:24037943..24044458	60B5-60B5

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TCTGCACATGAGAGTGGGC	CTGATCTTTCCGAAGCCTCCC

Images

Construct Design Sequences

sgRNA sequence	TAAAATGGATAATTCAAGCGCGG
sgRNA2 sequence	TTATAATAAAGTGTATTCTGCGG

 

left_200bp	right_200bp
TTGTCAAAAAATAAAAATTAAAATATGAAAACAGTTCAATATTTATATTAAAGCCTGTTTCAAAGCTTCCATCGTTCCGAATCAAAGCAATTGCACAGACTTCTGCATGCGTTAGCCATTGAGCCCTAGCGCCTCCACTCTGGTCACACTGCAACTGTTGCACGGCTGCAAATCGCAAAGCAAAATAAAATGCACCGCGC	CTGCGGTACGAGATTTTTACCACAGCGGAAACGCGACTTGGTCAAGTTAAATATTTCATTCTTTATTTCTACTGAAAATACAAATTATGTAGAAAATTCTCATACAAATTGGCGATCTCAACAGGATACGTGTTTCCATCGCTGTTTCATGCATGCCTTGTTTGTTAAATGCAATGCACAGTGCTCGTACAGTTTATTGG

 

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