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CRIMIC CR70821

Gene mfr

CG5747

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p0	3L:8905918..8912671	66E3-66E3

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TAGATGAGGAGCTGCAGGAG	GGGGTTATATCGATGGGACTGG

Images

Construct Design Sequences

sgRNA sequence	AAATTAAAGCTATACATATATGG
sgRNA2 sequence

 

left_200bp	right_200bp
AATATGGATAAGTTGCCATCCTTTGGTTACAAATTGGTTCCCGAACATGTGGAAACCCGCTCCCTTTGCCGCGATGATTTTCCAAATGTCGAACAGGTAAGTTAAGCAACCAAGTGTAGGTGCAAACCTATCCCAATCCAATGACTCACTCACATAGTTAGAATATCTAAAAATAGCATGGCCAAATTAAAGCTATACAT	ATATGGCCAAAAAGTGTCTAGACAGTCTCTATCAATATTCAACTAATCTGTGAGAAATATCTTGATATATGTTGATAAAACATATTACGTTTTATTTTCGATATAAAAAAGTATTATTTTTATTTGATATATATTTATAATAAACCAAACTACTAATGCAACCCATTGTCCAGGGCAAACTcCAACTGTGGATAGAGCTG

 

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