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CRIMIC CR70822

Gene LanB1

CG7123

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	2L:7811438..7820771	28C4-28D1

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CAGCTACAGGACTAGTTGGAAAATGC	ACATTGCAGTGTTGGTTTTCGC

Images

Construct Design Sequences

sgRNA sequence	CAACTGATTAAAGTCCCGGCCGG
sgRNA2 sequence	AGGCAGTGCTATACGTAGATTGG

 

left_200bp	right_200bp
TTGGTGGAATTCATAAATGTTGATCACTGTTAATTTGGAGAAGTGATGAAAACTGATACTTGAATCACCAAACTTTTTTTTGATAATTTTTACAAACTAAAATGAATTTTTTTTAGATTTTCATACTGAGGATCACGTGGACAAGTGTAAGAGATCCCCTTAAAAAACGAACGAAAAGAAAAGCAACTGATTAAAGTCCC	GATTGGGAAACGTAAGTAATGCAGTTTTAATACTACTACAAGCAAATTAATAAAATCAGTAATATTTTTTAGATCCATTTGCGTGGCCATCTAAATTGCCATATTCATGAACAAATAAGCAAATTGCGAAATACGAGAATCAAGTAAAGAACTACAAGAACAACTAGGAAGGCAACAGTTATTATGTAACCACTGTTAAG

 

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