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CRIMIC CR70824

Gene HDAC4

CG1770

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p0	X:13262687..13285632	11E8-11E9

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	ATGCTAATGGCAGTGTGGG	GATTGTTGTTGCTGCTGCTAGTG

Images

Construct Design Sequences

sgRNA sequence	TTAAAAAGACCTGTGCGACATGG
sgRNA2 sequence

 

left_200bp	right_200bp
AACAAAAATGAGAAATAAATAAATCACAACAGACGACTAAGTAAAAGAGAATTGAAATGAAATGAAATTATATATGTATGTATGTGGTTAGGTAGTATATGTATGCATACATTATATTATCGTGCCGGAAGTGTCGGAAGCGGCTTGTGCGATGACTAAGGCCATCATCGTTAACACTATGTATTTCTATATAGCCATGT	CGCACAGGTCTTTTTAATGTTCCAGAAAACGACTATTTCGCCAGGCACTCACATATGTACACATCATATACATCAATCTATGTCGTTATAGCAGCTAGATTTCGAATTAGTCATTCGGTATCTGTATAACTTTGAAATGCGCATAGATTAATCAGAATTTGGTATAAGTAATTAATGCCTTATTATCAATACAAACCTAT

 

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