CR70826

CRIMIC CR70826

Gene Fer3HCH

CG4349

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p0	X:13107382..13108194	11D11-11D1

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TGGATCGTATCCGATCCATCGACA	GAGTTACTATCCAATACCATCCACACAATG

Images

Construct Design Sequences

sgRNA sequence	ATAGTACGTTCAATACACGTAGG
sgRNA2 sequence	TGAAGAATACGTATTATAATTGG

left_200bp	right_200bp
CAAAAGACATGGCGTGGTGCTTCCGCGACATTCGGCGCCACATGTGCATGCTGGTGCGTCAGAACTTCGCCAAAAGTTGCGAGAAGAAGCTAAACGATCAGATCAACATGGAGTTGAAGGCATCCCACCAGTATCTGGCCATGGTAAGGTTCCGCAGTCAATAGGGAATATATATATATGCATATAGTACGTTCAATACA	TAATTGGCAAACCCATGTGTGTTCAGTAAAGATTCGTTTTTTGGCCGATCAATTGTTTTTTTTTTTTTTTTTTTGCTGCCACATTATCAAGTGGCTGGCGTAAAACTAACTCTATAAATATACTCGCTTATCGGCTCCAAAAGTTGCGCAAGATTCTAATTGAAATCGACACCGAAACTGAGCCCAAAAACAAAGCTTCA