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CRIMIC CR70828

Gene CstF50

CG2261

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	3R:31735503..31736963	100D1-100D

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GTCTTGCGGTTTACATTGTAGCG	GACTTAAGACTTGTGCGCTCAATG

Images

Construct Design Sequences

sgRNA sequence	TTTCGATAACGATAACGGCACGG
sgRNA2 sequence	TTTACTGATTATTTGCTCTTCGG

 

left_200bp	right_200bp
ATTGTTGAGAGGTTTAATTAATGAATCAAACAATGTTTAAAATCAATTGAGGGGACCAAAACAATTTTAAAACCGCACGTGTTGCAGCTGTTAGGAGTGACCGTGGACCGTAAAATACCGAAACCCTGATTTCAGTATTTCAATACCGCATAGCTAGAGATGACATACCGGCAAGATAAATGTTTTCGATAACGATAACG	AGCAAATAATCAGTAAACGGATGAAGCTACACCAATCGAATAAAACTTTAAGGAATAAAATATGTACACGTTCATTATTCAATTATTGTGATTGGCTGTGGGGGTTTTAAAGTGTATCGATTTGTGATTTTATCAGAATTAATGAAATATGTTTCAAACGCTTTTAAAAATAATTAATCAGTAAAATTTTCTTTATTAAA

 

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