CR70828

CRIMIC CR70828

Gene CstF50

CG2261

Information

Donor Location Cytology FBti Stock number
gRNA_int200_pM37_Kozak3R:31735503..31736963100D1-100D

PCR Verification

PCR check relative to sgRNA sequence

HA-L HA-R Forward Reverse
OK OK GTCTTGCGGTTTACATTGTAGCG GACTTAAGACTTGTGCGCTCAATG

Images

Construct Design Sequences

sgRNA sequence TTTCGATAACGATAACGGCACGG
sgRNA2 sequence TTTACTGATTATTTGCTCTTCGG
 
Amplicon PCR 5' Left Amplicon PCR 5' Right Amplicon PCR 3' Left Amplicon PCR 3' Right
 
Amplicon 5' Amplicon 3'
 

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