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CRIMIC CR70829

Gene Zip99C

CG7816

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p1	3R:29863701..29868895	99C4-99C5

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	AGGAGAAAATGGCTAAGGAGGG	GTGGATAGTGGAGGGCAAAGG

Images

Construct Design Sequences

sgRNA sequence	TGACTAGAAGGATCTTTAGGAGG
sgRNA2 sequence	TTCCTAGTGTTCGAATAGCATGG

 

left_200bp	right_200bp
AATTGAACTTGAATCACATCTCACCCTACAGCTGCAGATTCAAAACTCCTGCGAGTGCTTCTGAGTTTCGCGGTCGGCGGTCTGCTGGGCGATGTGTTCCTTCACCTGTTGCCAGAAGCCTGGGAGGGCGATAATCAAGGTGAGTTGCGGGGAAATCCAAGTTGTAAAATCATCTCCATAATATTGGTTTTCCTCCTAAA	TATTCGAACACTAGGAACTTTAGCTGACAGCAGTATAAGAAGAAAATTGAAACCAGTTTTTAGTTTGTTCTAAGATTGTCATTGGCAGTTTTAAATTAACATGAATTTGTTGTACTTTATGGGATTCGACATCCTGTCTGCGTTATGTAATTATAGCCAATATGAATGATATTTAATCTATTATTAATGATTACGTATTA

 

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