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CRIMIC CR70831

Gene Col4a1

CG4145

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p2	2L:5029609..5037279	25C1-25C1

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TTAATGATTAAAACCCAGTTATGGCCTAAA	GTTCATTTCGATTGTAGTGTTTCACGG

Images

Construct Design Sequences

sgRNA sequence	TGGACATAGCTCTCTTCGATTGG
sgRNA2 sequence

 

left_200bp	right_200bp
CCATTCAGAAAGAGCAAATATTAGTTTTTAAATTAATATTTTAGATATCAGCAATACAAAAATACATAATTTAAAACATCACATTTGTTCAATTTTTTTTTAATTTTTCGAAGCGCGATCTCTTAAAAACTTGTAAGCTCTCTCTTAAGCGCCTTTAGCAAACTCTCTTGAATTGACGGCTTAAGAGAGAGCTGCCAATC	GAAGAGAGCTATGTCCAATTAAATAGCTAGACTGATAAGACGAAGCACAGTTCCTAACCCTCCTGATCTTCGTCGACTTCTTTGCAGGCTGCTATACGCCGCTGTGATCGCGGGAGCGTTAGTCGGTGCCGACGCTGTAAGATAACCGTAGCCCATGCCATCCATCTGGACATTGAATAATATACTCCTTTTCCCCGCAG

 

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