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CRIMIC CR70833

Gene cer

CG10460

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p0	2R:19396215..19396772	56D3-56D3

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CGAAGTGGCATCTCTGATCAGC	CCTTCGTTCATTGAACCAAAACC

Images

Construct Design Sequences

sgRNA sequence	TTCTTGTCGAACTTGGACTATGG
sgRNA2 sequence	TTGAGATTCTGTTAATTTGGCGG

 

left_200bp	right_200bp
TTCTTTGTTGTGTTTATATGTTGTGACTCAGTGCGAAACCGTGATAAAACCTGAGAATTGAAACGAAATTTAGTCGGTCACCAAAATTACAAGTGTCAAGATGTCCCTGGTTTCAGATGAGGAGTGGGTGGAGTACAAGGTAGGCCCAATATGTCCTAGCAAGCGACGCAATATTTAGAAATCACTTGATTCCACCATAG	AATTAACAGAATCTCAAGTGAATCTCATTTTAAATAGACATTTCGTAAAGACACTAATCTCTCTGTAATTCTATAAATATGCATTTGAATTTTTAAATCTTTATCGTTCAACCACCCCCAAATAAACAATAAATCAGCTGTTTTGTACATTTTAAAGATTTTGGGATTTTTGTGTTCTGGCCACTTTGGCTGGGAGAAAA

 

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