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CRIMIC CR70839

Gene Bap55

CG6546

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p1	2R:17429353..17430963	54B7-54B7

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CATGCTATATGGCGGCGAC	ACCCAGAGTTCACTCCATATCC

Images

Construct Design Sequences

sgRNA sequence	GCTGGTCAGCATCTAGAGTAGGG
sgRNA2 sequence	GTCGAGCGGAAGTGTCCGTAAGG

 

left_200bp	right_200bp
TGCGAGTGGGCTACGCACAGGAGGATTCGCCCAAGGCGGAGATACCCTCGGTTGTGGGGATTGGAGCAGCTCCAGAGACCAATCTCGATCCGGAAACTAAAACAGACAACAATGCCACGCCAAATAGTTAGTTTCTACATCGCCTTTATTTAGTTTATGGCTTTTAAAAACCCGGAAAAACCGGCAATCCCTACCCCTAC	GTAAGGACAGCGCAATCGAATTCCAATTTTAATTTTAAAGACGCAACTCTATGTAATAATATAAATGTGAAATGTGAAAAGTGTCCCATTATTCAGAAGACATCTGAGCGTTTGTTTAGGAATAGATTGCATTTGATTACGGAAATGTTTTTAGTTACACACATATGTATATATTTACTGCGACGCTGACGCGGACGTTA

 

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