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CRIMIC CR70841

Gene Agpat2

CG17608

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	2L:8449691..8463994	29C5-29D1

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CGCACTTCAAACAATCTCGTAATAGTC	CTCGCTTAGTCGAAAAAAGCTC

Images

Construct Design Sequences

sgRNA sequence	TTTAGAAAAAGGATCCTTTGCGG
sgRNA2 sequence	GATAGGTTACGATCAAACTCAGG

 

left_200bp	right_200bp
TAATGGAGATAAATTTAAATGAGCTTAGTCACGAATGTTGATTTTCACTTTAAGTTTGTAGAAACCCAAGCATTAAATGTTCAATACAAAGCACAAGATTTCGAAATATATTCTCCTAGCTTAAATCATAAATTATTAAGAAATTATTATCAGTACCCATTTAATTTAAATCTTTGTTGTTATTTTAGAAAAAGGATCCT	CTCAGGTTACAAAAAAGTTACACCCAAACGTTTCGGGTTTAAATTTACAATCGATCCACAAGATTTGCCTATCCATGGATGAGATGAATCAAAGGCAACAGAATGCCGTTACTTGAGGGACGTAAAAACGTTGGCCTAGTATTAAGTTACACAATATCACTCCATGGGTGAATTTTCGATTAATATTAACTGTTTTACCT

 

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