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CRIMIC CR70842

Gene Eglp2

CG17664

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p0	2R:23603722..23616754	59F4-59F4

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CTGCCAGTGCGATTTGGTC	GCGTGCTTGTAGATCACCGAG

Images

Construct Design Sequences

sgRNA sequence	GATGATAACCTTTCTTTAAATGG
sgRNA2 sequence

 

left_200bp	right_200bp
AGATACAGTATTAGATATATCTTATTTTGTAATCCACCTTAATGTCTGTTTGTCCAAGTAATACCGATACAGATAAGACTGTCTGCTCTTAAGAAATATCAAATTGACGATCCGCTATGCTAAAATTCCATTCACTATCTGTTTTCAATTTATCAGTCAAATGCACTTGACTTTCTCGGTATCGATGATAACCTTTCTTT	AAATGGCATATAAACAGTTTTAATATAGACATTTTAGCCATCGCATGAAATGCAATCAATAGGTATCAAAAAAATGTTCTTAATAAACAATTGTAAATCCCCCAACAGGGACAACTGACTGGGGCTAGTATGAATCCAGTCCGATCCTTTGCTCCGGCCATTTGGAACGGATTCTGGGATGACCACTGGATCTACTGGGT

 

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